Purification and characterization of a hygromycin B phosphotransferase from Streptomyces hygroscopicus
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چکیده
منابع مشابه
Purification and characterization of a hygromycin B phosphotransferase from Streptomyces hygroscopicus.
A hygromycin B phosphotransferase activity from Streptomyces hygroscopicus has been highly purified by ammonium sulphate fractionation followed by affinity column chromatography through Sepharose-6B-hygromycin-B. The combined active fractions showed a single protein band (41 kDa) when subjected to polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate. When gel electropho...
متن کاملEnzymatic modification of hygromycin B in Streptomyces hygroscopicus.
Antibiotic producing-organisms generally exhibit resistance to the antibiotics that they produce1). Such resistance mechanisms may take the form of target site modification, as is the case with erythromycin2) and thiostrepton3), or enzymatic modification of the antibiotic itself, as has been shown for a number of aminocyclitols4) The existence of these resistance mechanisms in producing organis...
متن کاملAn O-phosphotransferase catalyzes phosphorylation of hygromycin A in the antibiotic-producing organism Streptomyces hygroscopicus.
The antibiotic hygromycin A (HA) binds to the 50S ribosomal subunit and inhibits protein synthesis in gram-positive and gram-negative bacteria. The HA biosynthetic gene cluster in Streptomyces hygroscopicus NRRL 2388 contains 29 open reading frames, which have been assigned putative roles in biosynthesis, pathway regulation, and self-resistance. The hyg21 gene encodes an O-phosphotransferase wi...
متن کاملLarge-scale purification and acute toxicity of hygromycin B phosphotransferase.
OBJECTIVE To provide the acute toxicity data of hygromycin B phosphotransferase (HPT) using recombinant protein purified from E. coli. METHODS Recombinant HPT protein was expressed and purified from E. coli. To exclude the potential adverse effect of bacteria protein in recombinant HPT protein, bacterial control plasmid was constructed, and bacteria control protein was extracted and prepared ...
متن کاملBiochemical basis of resistance to hygromycin B in Streptomyces hygroscopicus--the producing organism.
Hygromycin B, an aminocyclitol antibiotic that strongly inhibits both 70S and 80S ribosomes, is synthesized by Streptomyces hygroscopicus. Ribosomes from this Gram-positive mycelial bacterium are inhibited in vitro by the antibiotic. In contrast, the streptomycete is highly resistant to the drug in vivo since it possesses hygromycin B phosphotransferase activity. This enzyme has been shown by g...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1987
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1987.tb10618.x